Napredna ekstrakcija in formulacija funkcionalnih taninskih prahranskih dopolnil z blagodejnimi zdravstvenimi učinki/Advanced Extraction and Formulation of Functional Tannin Food Supplements with Beneficial Health Effects

Glavni cilj predlaganega projekta je pridobivanje in čiščenje taninov iz rastlinskih materialov (kostanjev les, hrastov les) in nato razvoj končnega izdelka, ki bo uporabljen kot prehransko dopolnilo. Globalna težnja je bila napram uporabi naravnih bioaktivnih sestavin v živilski, farmacevtski in zlasti fitofarmacevtski industriji.V prvem delu projekta bo izvedena ekstrakcija iz naravnih virov (kostanjev les, hrastov les itd.) S konvencionalnimi ekstrakcijskimi tehnikami in superkritičnim fluidom, ekstrakcijo z mikrovalovi in ​​u ltrazvočno ekstrakcijo. Raziskave bodo osredotočene na izolacijo glavnih taninov npr. veskalin, kastalin, veskalagin, kastalagin, grandinin, roburin E, roburin D itd. V drugem delu bo izvedena formulacija izdelka na osnovi bioloških produktov. Delo se bo osredotočilo tudi na formuliranje dobljenih ekstraktov v posebne oblike, kot so praški, zasnovani za specifično uporabo. Tretji del projekta bo preučeval biološko aktivnost čistih ekstraktov in izoliranih taninov. Za dokazovanje biološke aktivnosti in biološke kompatibilnosti posameznih izoliranih snovi s tarčnimi celicami bodo izvedeni testi in vitro s človeškimi celicami V zadnjem delu bodo predstavljene potencialne tehnološke sheme in procesni parametri za izvedbo razširitve procesa na industrijsko merilo. Za identifikacijo izoliranih spojin in formuliranih izdelkov bodo uporabljene sodobne analitske metode, kot so HPLC, HS-GC-MS, LC-MS / MS, NMR itd. Postopki, ki se uporabljajo za ekstrakcijo, čiščenje in formulacijo taninov v končno obliko funkcionalnega prehranskega dopolnila, bodo povečali konkurenčnost industrijskega partnerja Tanin Sevnica d.d v svetovnem merilu.The goal role of the proposed project is to extract and purify tannins from plant materials (chestnut wood, oak wood) and afterward develop a final product that will be used as a dietary supplement.  There has been a global tendency towards the use of natural bioactive components in the food, pharmaceutical and especially the phytopharmaceutical industries. In the first part of the project, extraction from natural sources (chestnut wood, oak wood etc.) using classical extraction techniques and supercritical fluis augmented by microwaves and ultrasound extraction will be investigated. Research will be focused on the isolation of main tannins e.g. vescalin, castalin, veskalagin, kastalagin, grandinin, roburin E, Roburin D etc..In the second part the bio-based product formulation by design will be performed. Work will also focus on the formulation of the obtained extracts into specific forms like powders, designed for a specific use. The third part of the project will study biological activity of crude extracts and isolated tannins. In vitro tests using human cells will be carried out to prove the bio activity and bio compatibility of the respective isolated substances with targeted cells. In the final part the potential technological schemes and process parameters for scale up will be presented. For the identification of isolated compound and formulated products modern analytical methods like HPLC, HS-GC-MS, LC-MS/MS, NMR etc.) will be applied. Processes used for extraction, purification and formulation of tannins into the final form of a functional supplement product will increase the competitiveness of the industrial partner Tanin Sevnica d.d on a global scale.